It has been confirmed that nephropathy is one of the secondary complications of diabetes mellitus; diabetic nephropathy has been associated with progressive oxidative stress. The present study was conducted to investigate whether biotin can reduce kidney injury and oxidative stress in kidney tissues in diabetic mice type 1. Mice were divided into 3 groups, the first group served as control group. Diabetes type 1 was induced in the second and third groups by a single dose of 150 mg/kg given intraperitoneally in sodium citrate buffer at pH 4.5. The second group remained as untreated diabetes group and the third group received 15 mg/kg daily oral dose of biotin for 12 days. Biochemical analysis results showed increasing in glucose, BUN levels and no change in creatinin levels, increasing in glomerular areas and decreasing in cellularity in glomeruli in both diabetic groups. Whereas,histopathological results showed severe alterations in untreated diabetic group represented by distorted glomeruli, inflammatory cells, giant macrophages and intense immunoreaction response for acrolin gene expression that an indicator for oxidative stress.Moreover, histopathology of treated diabetic group with biotin showed less pathological changes and slight immunoreactions response for oxidative stress.
Keywords: Diabets-Nephrotoxicity-Streptozotocin- Acrolin –Mice.
Posted by: badr, prof, ksu, Saudi Arabia (27-Dec-2014)