10th Brazil School for Single Particle Cryo-EM

  • 2022

Description

The 10th Brazil School for Single Particle Cryo-EM is an hands-on workshop in the field of Cryo-electron microscopy (cryo-EM).

10th Brazil School for Single Particle Cryo-EM covers topics such as:

  • Properties of the Fourier transform
  • The Fourier transform in 1D - 2D - 3D
  • (Automatic) Particle selection
  • Specimen preparation for single-particle cryo-EM
  • CTF correction
  • Optimizing the EM data collection
  • Alignment using correlation functions
  • Filtering and data normalization
  • Multivariate statistics and classification
  • Multi-reference alignments
  • Three-dimensional reconstruction
  • Angular reconstitution
  • Alignment refinements
  • Projection matching
  • 3D resolution criteria
  • 3D refinements
  • Refining towards different 3D structures simultaneously ("4D" analysis)
  • 3D fitting of known X-ray structures (Chimera, Coot)
  • Parallel processing
  • Using image processing software (IMAGIC, Frealign)

Past Events

Important

Please, check "Brazil School for Single Particle Cryo-EM" official website for possible changes, before making any traveling arrangements

Event Categories

Science: Engineering, Laboratories, Life Sciences & Biology, Physics

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