The 10th Brazil School for Single Particle Cryo-EM is an hands-on workshop in the field of Cryo-electron microscopy (cryo-EM).
10th Brazil School for Single Particle Cryo-EM covers topics such as:
- Properties of the Fourier transform
- The Fourier transform in 1D - 2D - 3D
- (Automatic) Particle selection
- Specimen preparation for single-particle cryo-EM
- CTF correction
- Optimizing the EM data collection
- Alignment using correlation functions
- Filtering and data normalization
- Multivariate statistics and classification
- Multi-reference alignments
- Three-dimensional reconstruction
- Angular reconstitution
- Alignment refinements
- Projection matching
- 3D resolution criteria
- 3D refinements
- Refining towards different 3D structures simultaneously ("4D" analysis)
- 3D fitting of known X-ray structures (Chimera, Coot)
- Parallel processing
- Using image processing software (IMAGIC, Frealign)