Mouse Models: Genetics Breeding and Experimental Design 2016 is a course dedicated to designing, undertaking analysis and reporting experiments using mice.
Mouse Models: Genetics Breeding and Experimental Design 2016 covers topics such as:
- Gene & strain nomenclature
- Overview of mouse genetics & strains
- Breeding strategies for maintaining inbred, outbred and genetically altered lines
- Aspects of good colony management
- Conditional and inducible transgenesis
- Establishing and maintaining transgenic lines and gene targeted lines
- Genotyping
- CRISPR/Cas9 and emerging technologies for generating GA colonies
- Workshops on designing breeding programs
- Quality control strategies for mouse lines
- Electronic tools for colony management
- Cryopreservation
- Pilot experiments
- Experimental design:
- The experimental unit
- Randomisation and blinding
- The 3Is
- Calculating experimental numbers
- Experimental controls
- Ethics and the 3Rs
- Standardisation and variability: effects of the environment, time and health status on experimental results
- Statistical analysis
