Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs 2016) is a conference dedicated to current theory and best practices with regard to derivation and culture of human induced pluripotent stem cells (hiPSCs).
Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs 2016) covers topics such as:
- Key advantages, disadvantages and critical elements of different approaches to somatic cell reprogramming
- Key principles of good cell culture practice (GCCP) and aseptic processing including the role of antibiotics
- Critical aspects for successful preservation and storage
- Undifferentiated, fully reprogrammed iPSCs compared to partially reprogrammed iPSC colonies
- Undifferentiated morphology of confluent iPSCs ready for passaging
- Evaluate the recovery of iPSCs and identify viable cells
- The importance of core QC and potential consequences of failing to carry it out
- Critical aspects in the passaging and maintenance of undifferentiated iPSCs
- Different capabilities of each method of determining potential pluripotency